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      <image:title>Journal - A Winter Wedding: Jennifer &amp; Scott</image:title>
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      <image:caption>Credits: Images on this site belong to McKenzie Powell Floral &amp; Event Design.</image:caption>
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      <image:caption>Migratory neural crest cells undergo EMT and give rise to a diverse array of structures in the developing vertebrate embryo.</image:caption>
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      <image:title>Snail2 targets during neural crest cell EMT and migration</image:title>
      <image:caption>Premigratory neural crest cells in the dorsal neural tube of the chick hindbrain express Cadherin-6B (green) along their plasma membranes, with a high concentration apically (facing the neural tube lumen) and laterally at their edges. As neural crest cells undergo their epithelial-to-mesenchymal transition and begin to leave the neural tube, they express the transcription factor FoxD3 (purple cell nuclei) and gradually lose Cadherin-6B. Migratory neural crest cells will later give rise to a diverse array of derivatives in the chick head, including the craniofacial skeleton and sensory ganglia.</image:caption>
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      <image:title>The Neural Crest</image:title>
      <image:caption>In ovo electroporation of morpholino antisense-oligonucleotides (MOs) into the neural tube targets a specific mRNA in the premigratory neural crest of the chick midbrain. MOs are employed in order to knock-down/inhibit translation of the mRNA of interest. MOs are labeled with a fluorophore (here lissamine, red) to allow for visualization with a fluorescence microscope.</image:caption>
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      <image:title>Neural crest cell interactions and the creation of the cranial ganglia</image:title>
      <image:caption>Electroporation of a morpholino (MO, red) to knock-down αN-catenin translation in migratory neural crest cells impacts the later formation of placode cell-derived neurons (labeled with an antibody to Tubb3, green) within the trigeminal ganglion (A, arrows), with no effect on the contralateral control side of the same embryo (B). e, eye; TG, trigeminal ganglion. Scale bar in (A) is 100 µm and applicable to (B). Adapted from Wu et al., 2014.</image:caption>
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